WP 4 Molecular regulation of commensal growth and fungal factors

In WP4, we will investigate factors of C. albicans that (a) have been identified by our partners in Jena and Paris as associated with the “compath” shift, (b) factors already known to play distinct regulatory roles in the commensal phase (Efg1, Efh1, Wor1) and (c) a novel potential commensal effector protein (Ece1). We will explore the molecular mechanisms, by which these key regulators allow commensal growth of C. albicans in the gut. We will generate C. albicans strains with conditional, tetracycline-dependent expression of Efg1, Efh1 and Wor1. We will also develop strains that allow in vivo FLP-mediated conditional deletion of regulator genes in the gut to analyze their influence at different stages of colonization. Strains will be fluorescently labeled and assayed in competition assays with wild-type strains to uncover how regulator levels determine colonization and fungal morphology (e.g. the GUT phenotype).

References

Buerth C, Tielker D, Ernst JF (2016) Candida utilis and Cyberlindnera (Pichia) jadinii: yeast relatives with expanding applications. Appl Microbiol Biotechnol 100, 6981-6990. PubMed Springer Link

Lassak T, Schneider E, Bussmann M et al. (2011) Target specificity of the Candida albicans Efg1 regulator. Mol Microbiol 82, 602-618.
PubMed Open Access PDF

Schaekel A, Desai PR, Ernst JF (2013) Morphogenesis-regulated localization of protein kinase A to genomic sites in Candida albicans. BMC Genomics 14, 842.
PubMed Open Access PDF

Stichternoth C, Fraund A, Setiadi S et al. (2011) Sch9 kinase integrates hypoxia and CO2 sensing to suppress hyphal morphogenesis in Candida albicans. Eukaryot Cell 10, 502-511.
PubMed Open Access PDF

Swidergall M, Ernst AM, Ernst JF (2013) Candida albicans mucin Msb2 is a broad-range protectant against antimicrobial peptides. Antimicrob Agents Chemother 57, 3917-3922.
PubMed Open Access PDF

Szafranski-Schneider E, Swidergall M, Cottier F et al. (2012) Msb2 shedding protects Candida albicans against antimicrobial peptides. PLoS Pathog 8, e1002501.
PubMed Open Access PDF

Related partners

Heinrich Heine University Düsseldorf

Heinrich Heine University Düsseldorf

Address

Heinrich Heine University Düsseldorf
Universitätsstr. 1/26.12
40225 Düsseldorf, Germany

Principal Investigator

Scientists

Christoph Bürth

Christoph Bürth

+49 211 81-15488

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Hans Knöll Institute Jena

Hans Knöll Institute Jena

Address

Leibniz Institute for Natural Product Research and Infection Biology
Hans Knöll Institute
Adolf-Reichwein-Str. 23
07745 Jena, Germany

Coordinator and Principal Investigator

Bernhard Hube

Bernhard Hube

+49 3641 532 1401

www.leibniz-hki.de

Scientists

Katja Graf

Katja Graf

+49 3641 532 1218

Antonia Dräger

Antonia Dräger

+49 3641 532 1595

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Institut Pasteur Paris

Institut Pasteur Paris

Address

Institut Pasteur
25, rue du Docteur Roux
75015 Paris, France

Principal Investigator

Christophe d’ Enfert

Christophe d’ Enfert

+33 1 40 61 32 57

www.pasteur.fr

Scientists

Marie-Elisabeth Bougnoux

Marie-Elisabeth Bougnoux
Associate Professor

Natacha Sertour

Natacha Sertour
Technician

Murielle Chauvel

Murielle Chauvel
Technician

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Universidad Complutense de Madrid

Universidad Complutense de Madrid (USM)

Address

Universidad Complutense de Madrid (USM)
Avda. Ramón y Cajal s/n
28040 Madrid, Spain

Principal Investigator

Jesús Pla

Jesús Pla

+34 91 3941617

www.ucm.es

Scientists

Elvira Román

Elvira Román

Daniel Prieto

Daniel Prieto

+34 610 686818

Rebeca Alonso Monge

Rebeca Alonso Monge

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