WP 5 Protective yeasts
We will dissect the protective role of the commensal yeast P. jadinii to counteract C. albicans overgrowth and infection. Of note, the genome of this yeast has been sequenced and can be genetically manipulated. This offers the unique possibility to analyze the genetics of both a probiotic yeast and its antagonist C. albicans. Considering that antibiotic treatment is a major risk factor of disseminated C. albicans infection, the potential use of antibiotic-resistant probiotic yeasts to prevent translocation from the gut seems particularly attractive.
References
Arana DM, Nombela C, Pla J (2010) Fluconazole at subinhibitory concentrations induces the oxidative- and nitrosative-responsive genes TRR1, GRE2 and YHB1, and enhances the resistance of Candida albicans to phagocytes. J Antimicrob Chemother 65, 54-62.
PubMed Open Access PDF
Galan-Diez M, Arana DM, Serrano-Gomez D et al. (2010) Candida albicans beta-glucan exposure is controlled by the fungal CEK1-mediatedmitogen-activated protein kinase pathway that modulates immune responses triggered through dectin-1. Infect Immun 78, 1426-1436.
PubMed Open Access PDF
Huertas B, Prieto D, Pitarch A, Gil C, Pla J, Díez-Orejas R (2017) Serum antibody profile during colonization of the mouse gut by Candida albicans: Relevance for protection during systemic infection. J Proteome Res 16, 335-345.
PubMed
New: Leonardi I, Li X, Semon A, Li D, Doron I, Putzel G, Bar A, Prieto D, Rescigno M, McGovern DPB, Pla J, Iliev ID (2018) CX3CR1+ mononuclear phagocytes control immunity to intestinal fungi. Science 359, 232-236.
Science
Prieto D, Correia I, Pla J, Román E (2016) Adaptation of Candida albicans to commensalism in the gut. Future Microbiol 11, 567-583.
PubMed
Prieto D, Román E, Alonso-Monge R, Pla J (2017) Overexpression of the transcriptional regulator WOR1 increases susceptibility to bile salts and adhesion to the mouse gut mucosa in Candida albicans. Front Cell Infect Microbiol 7, 389. PubMed Open Access
Prieto D, Roman E, Correia I, Pla J (2014) The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans. PLoS ONE 9 e87128.
PubMed Open Access PDF
Prieto D, Pla J (2015) Distinct stages during colonization of the mouse gastrointestinal tract by Candida albicans. Front Microbiol 5, 792.
PubMed Open Access
Román E, Correia I, Salazin A, Fradin C, Jouault T, Poulain D, Liu FT, Pla J (2016) The Cek1-mediated MAP kinase pathway regulates exposure of a-(1,2) and β-(1,2)-mannosides in the cell wall of Candida albicans modulating immune recognition. Virulence 7, 558-577.
PubMed
Szafranski-Schneider E, Swidergall M, Cottier F et al. (2012) Msb2 shedding protects Candida albicans against antimicrobial peptides. PLoS Pathog 8, e1002501.
PubMed Open Access PDF
Thomas E, Roman E, Claypool S et al. (2013) Mitochondria influence CDR1 efflux pump activity, Hog1-mediated oxidative stress pathway, iron homeostasis, and ergosterol levels in Candida albicans. Antimicrob Agents Chemother 57, 5580-5599.
PubMed Open Access PDF
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Universidad Complutense de Madrid (USM)
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Universidad Complutense de Madrid (USM)
Avda. Ramón y Cajal s/n
28040 Madrid, Spain
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Heinrich Heine University Düsseldorf
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Heinrich Heine University Düsseldorf
Universitätsstr. 1/26.12
40225 Düsseldorf, Germany