WP 5 Protective yeasts

We will dissect the protective role of the commensal yeast P. jadinii to counteract C. albicans overgrowth and infection. Of note, the genome of this yeast has been sequenced and can be genetically manipulated. This offers the unique possibility to analyze the genetics of both a probiotic yeast and its antagonist C. albicans. Considering that antibiotic treatment is a major risk factor of disseminated C. albicans infection, the potential use of antibiotic-resistant probiotic yeasts to prevent translocation from the gut seems particularly attractive.

References

Arana DM, Nombela C, Pla J (2010) Fluconazole at subinhibitory concentrations induces the oxidative- and nitrosative-responsive genes TRR1, GRE2 and YHB1, and enhances the resistance of Candida albicans to phagocytes. J Antimicrob Chemother 65, 54-62.
PubMed Open Access PDF

Galan-Diez M, Arana DM, Serrano-Gomez D et al. (2010) Candida albicans beta-glucan exposure is controlled by the fungal CEK1-mediatedmitogen-activated protein kinase pathway that modulates immune responses triggered through dectin-1. Infect Immun 78, 1426-1436.
PubMed Open Access PDF

Huertas B, Prieto D, Pitarch A, Gil C, Pla J, Díez-Orejas R (2017) Serum antibody profile during colonization of the mouse gut by Candida albicans: Relevance for protection during systemic infection. J Proteome Res 16, 335-345.
PubMed

Prieto D, Correia I, Pla J, Román E (2016) Adaptation of Candida albicans to commensalism in the gut. Future Microbiol 11, 567-583.
PubMed

Prieto D, Román E, Alonso-Monge R, Pla J (2017) Overexpression of the transcriptional regulator WOR1 increases susceptibility to bile salts and adhesion to the mouse gut mucosa in Candida albicans. Front Cell Infect Microbiol, in press.
Open Access

Prieto D, Roman E, Correia I, Pla J (2014) The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans. PLoS ONE 9 e87128.
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Prieto D, Pla J (2015) Distinct stages during colonization of the mouse gastrointestinal tract by Candida albicans. Front Microbiol 5, 792.
PubMed Open Access

Román E, Correia I, Salazin A, Fradin C, Jouault T, Poulain D, Liu FT, Pla J (2016) The Cek1-mediated MAP kinase pathway regulates exposure of a-(1,2) and β-(1,2)-mannosides in the cell wall of Candida albicans modulating immune recognition. Virulence 7, 558-577.
PubMed

Szafranski-Schneider E, Swidergall M, Cottier F et al. (2012) Msb2 shedding protects Candida albicans against antimicrobial peptides. PLoS Pathog 8, e1002501.
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Thomas E, Roman E, Claypool S et al. (2013) Mitochondria influence CDR1 efflux pump activity, Hog1-mediated oxidative stress pathway, iron homeostasis, and ergosterol levels in Candida albicans. Antimicrob Agents Chemother 57, 5580-5599.
PubMed Open Access PDF